Evaluation of the antioxidant capacity and bioactive compounds of banana passionfruit (Passiflora mollissima) and black cherry (Prunus serotina)
DOI:
https://doi.org/10.24265/horizmed.2021.v21n3.08Keywords:
Antioxidants, Phenols, Flavonoids, Prunus serotinaAbstract
Objective: To determine the content of bioactive compounds and antioxidant capacity of the banana passionfruit (Passiflora mollissima) and black cherry (Prunus serotina). Materials and methods: An analytical, experimental, longitudinal and prospective study. The black cherries and banana passionfruits were collected in the Cusco, Moquegua and Arequipa regions. The content of phenols and flavonoids were
determined using the Folin–Ciocalteu reagent and aluminum chloride method, respectively. The antioxidant activity was evaluated using the ferric reducing ability of plasma (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and thiobarbituric acid reactive substance (TBARS) techniques. Albino rats classified into a negative control group, a positive control group and four experimental groups were used to study the hepatoprotective effect of the fruits. Results: Banana passionfruits from the Cusco region (Quechua) showed the highest concentration of total phenols (584.94 ± 134.62 mg GAE/100 g) and flavonoids (445.62 ± 7.94 mg QE/100 g). Concerning the DPPH radical, the IC50 value of banana passionfruits from the Arequipa region (Yunga) was found to be 0.41 ± 0.01 mg/mL. Banana passionfruits
from the Cusco region (Quechua) showed the highest FRAP value (8.38 ± 0.32 mmol Fe2+/100 g). Black cherries from the Arequipa region (Yunga) had the highest concentration of total phenols (181.81 ± 34.1 mg GAE/100 g) and flavonoids (205.18 ± 77.8 mg QE/100 g). They also showed a significant antioxidant activity regarding the DPPH (2.1 ± 0.01 mg/mL), while
the antioxidant capacity of black cherries from the Cusco region (Quechua), which was evaluated with the FRAP method, achieved a value of 1.59 ± 0.2 mmol Fe2+/100 g. The observed differences were statistically significant. Banana passionfruits showed a better hepatoprotective effect than black cherries.
Conclusions: Banana passionfruits from the Cusco region (Quechua) are an important source of antioxidant compounds and show a high antioxidant capacity (FRAP), while black cherries from the Arequipa region (Yunga) have a high content of antioxidant compounds and a higher antioxidant capacity (DPPH).
Downloads
References
Fehér J, Csomos G, Vereckei A. Free Radicals Reactions in Medicine. Springer-Verlag Berlin Heidelberg: Germany; 1987.
Hernández-García D, Wood CD, Castro-Obregón S, Covarrubias L. Reactive oxygen species: a radical role development?. Free Radic Biol Med. 2010; 49(2): 130-43.
Halliwell B, Gutteridge JMC. Free radicals in Biology and Medicine. 4th ed. Oxford University Press: United Kingdom; 2007.
Cascales M. La toxicidad del oxígeno. Estrés oxidativo. En: Estrés oxidativo, envejecimiento y enfermedad. España; 1999. pp 9-46.
Virgili F, Marino M. Regulation of cellular signals from nutritional molecules: a specific role for phytochemicals, beyond antioxidant activity. Free Radic Biol Med. 2008; 45(9): 1205-16.
Pryor W. Oxidants and antioxidants. En: Natural antioxidants in human health and disease. Academic Press: USA; 1994.
Olatunji TL, Afolayan AJ. Comparative Quantitative Study on Phytochemical Contents and Antioxidant Activities of Capsicum annuum L and Capsicum frutescens L. Sci World J. 2019.
Li C, Feng J, Huang W-Y, An X-T. Composition of Polyphenols and Antioxidant Activity of Rabbiteye Blueberry (Vaccinium ashei) in Nanjing. J Agric Food Chem. 2013; 61(3): 523-31.
Biazotto KR, de Souza Mesquita LM, Neves BV, Cavalcante Braga AR, Pereira Tangerina MM, Vilegas W, et al. Brazilian Biodiversity Fruits: Discovering Bioactive Compounds from Underexplored Sources. J Agric Food Chem. 2019; 67(7): 1860-76.
Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. Antioxidant therapy: a new pharmacological approach in shock, inflammation and ischemia/reperfusion injury. Pharmacol Rev. 2001; 53(1): 135-59.
Chen J, Jeppesen PB, Abudula R, Dyrskog SEU, Colombo M, Hermansen K. Stevioside does not cause increased basal insulin secretion or β-cell desensitization as does the sulphonylurea, glybenclamide: Studies in vitro. Life Sci. 2006; 78(15): 1748-53.
Wilson DW, Nash P, Buttar HS, Griffiths K, Singh R, De Meester F, et al. The Role of Food Antioxidants, Benefits of Functional Foods, and Influence of Feeding Habits on the Health of the Older Person: An Overview. Antioxidants (Basel). 2017; 6(4): 81.
Chaparro-Rojas DC, Maldonado ME, Franco-Londoño MC, Urango-Marchena LA. Características nutricionales y antioxidantes de la fruta curuba larga (Passiflora mollissima Bailey). Perspect Nutr Humana. 2014; 16(2): 203-12.
Guadalupe JJ, Gutiérrez B, Intriago-Baldeón DP, Arahana V, Tobar J, Torres AF, et al. Genetic diversity and distribution patterns of Ecuadorian capuli (Prunus serotina). Biochem System Ecol. 2015; 60: 67-73.
Luna-Vázquez FJ, Ibarra-Alvarado C, Rojas-Molina A, Rojas-Molina JI, Yahia EM, Rivera-Pastrana DM, et al. Nutraceutical Value of Black Cherry Prunus serotina Ehrh. Fruits: Antioxidant and Antihypertensive Properties. Molecules. 2013; 18(12): 14597-612.
Correia S, Gonçalves B, Aires A, Silva A, Ferreira L, Carvalho R, et. al. Effect of Harvest Year and Altitude on Nutritional and Biometric Characteristics of Blueberry Cultivars. J Chem. 2016; (1): 1-12.
Carrión Jara AV, García Gómez CR. Preparación de extractos vegetales: Determinación de eficiencia de metódica [Tesis]. Ecuador: Universidad de Cuenca. Facultad de Ciencias Químicas; 2010.
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybic-phophotungstic acid reagents. Amer J Enol Vit. 1965; 16: 144-58.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64(4): 555-9.
Brand-Williams W, Cuvelier M, Berset C. Use of a Free Radical Method to Evaluate Antioxidant Activity. Food Sci Technol. 1995; 28(1): 25-30.
Benzie IF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of «Antioxidant Power»: The FRAP Assay. Anal Biochem. 1996; 239(1): 70-6.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2): 351-8.
Langhans, W. Food components in health promotion and disease prevention. J Agric Food Chem. 2017; 66(10): 2287-94.
Connor AM, Finn CE, Alspach P. Genotypic and environmental variation in antioxidant activity and total phenolic content among Blackberry and hybrid berry cultivars. J Am Soc Hortic Sci. 2005; 130(4): 527-33.
Wojdyło A, Nowicka P, Laskowski P, Oszmiański J. Evaluation of Sour Cherry (Prunus cerasus L.) Fruits for Their Polyphenol Content, Antioxidant Properties, and Nutritional Components. J Agric Food Chem. 2014; 62(51): 12332-45.
Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E, García-Villanova B. Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Res Intern. 2011; 44(7): 2047-53.
Vasco C, Ruales J, Kamal-Eldin A. Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chem. 2008; 111(4): 816-23.
Jimenez M, Castillo I, Azuara E, Beristain CI. Antioxidant and antimicrobial activity of capulin (Prunus serotina subsp capuli) extracts. Rev Mex Ing Quim. 2011; 10(1): 29-37.
Liu M, Li XQ, Weber C, Lee CY, Brown J, Liu Rh. Antioxidant and antiproliferative activities of raspberries. J Agric Food Chem. 2002; 50(10): 2926-30.
Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. J Agric Food Chem. 2003; 51(3): 609-14.
Szeto YT, Tomlinson B, Benzie IFF. Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary and planning and food preservation. British J Nut. 2002; 87(1): 55-9.
Kosar M, Altintas A, Kirimer N, Baser KHC. Determination of the free radical scavenging activity of Lycium extracts. Chem Nat Compd. 2003; 39(6): 531-5.
Lim, YK, Lim TT, Tee JJ. Antioxidant properties of several tropical fruits: A comparative study. Food Chem. 2007; 103(1): 1003-8.
Zhou H-C, Wang H, Shi K, Li J-M, Zong Y, Du R. Hepatoprotective Effect of Baicalein Against Acetaminophen-Induced Acute Liver Injury in Mice. Molecules. 2018; 24(1): 131.
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Horizonte Médico (Lima)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Horizonte Médico (Lima) (Horiz. Med.) journal’s research outputs are published free of charge and are freely available to download under the open access model, aimed at disseminating works and experiences developed in biomedical and public health areas, both nationally and internationally, and promoting research in the different fields of human medicine. All manuscripts accepted and published in the journal are distributed free of charge under the terms of a Creative Commons license – Attribution 4.0 International (CC BY 4.0).
